Journal: bioRxiv

Article Title: Biocompatibility of KAPs-Depleted Residual Hair

doi: 10.1101/2024.04.29.591537

Figure Lengend Snippet: Hair biomaterial implants and their up-close interfacial interaction to the foreign body response cells and ECM components. Three representative images per group: 2 at lower magnification and 1 (boxed main) at higher showing clusters of immune cells (red) of mostly Mφ = macrophages and mast cells, and fibroblasts within the collagen ECM (blue).

Article Snippet: After microtome sectioning at a thickness of 5-mm and rehydration, samples were stained with Masson’s trichrome (MT, connective tissue stain, ab150686 kit, Abcam, Waltham, MA) to highlight and detect connective tissue and immune cell components (black/dark for cell nuclei, red for cytoskeleton and cytosol of cells, and blue for the collagen extracellular matrix (ECM)), dehydrated, mounted using Histomount (Thermo Fisher Scientific, Waltham, MA), and imaged using Cytation 5 Cell Imaging Multimode Reader (Agilent, Santa Clara, CA), and Primostar 3 with Excelis (Zeiss, White Plains, NY) at low and high magnifications.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Phosphorylated alpha-synuclein distribution in the colonic enteric nervous system of patients with diverticular disease

doi: 10.1016/j.ibneur.2025.02.009

Figure Lengend Snippet: Comparison of Age, Gender Distribution, and P-aSyn Immunoreactivity Across Control, Diverticulosis (Dose), and Diverticulitis (Ditis) Cohorts. P-aSyn IR: phosphorylated alpha-synuclein immunoreactivity.

Article Snippet: Briefly, after deparaffinization in xylol and rehydration in alcohol with descending concentrations, tissue sections were pretreated with citrate buffer (pH 6.0, 95°C, water bath) for 25 minutes, followed by overnight incubation with rabbit-anti-phosphorylated alpha-synuclein antibody (1/1000, D1R1R, Cell signaling Technology) in antibody diluent (Thermo Fisher Scientific, Carlsbad, USA).

Techniques: Comparison, Control